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Mitochondria in the L. edodes was separated and purified by stepped sucrose density gradient centrifugation. The activity of mitochondrial ATP synthase has been investigated during various illumination times at each wavelength within the range of 400 ㎚ to 700 ㎚. The stimulation of above activity increased by two times compared with nonilluminated control group when the illumination was given for 15 seconds at 470 ㎚ wavelength. The optimal pH and temperature of this light-induced mitochondrial ATP synthase were 7.5 and 54℃, respectively. The activity of this enzyme increased by 26%, 25% and 14%, respectively, when there were 1 mmole Fe^(3+), 0.5 mmole Fe^(2+) and 5 mmole SO₄^(2+) ion, and was inhibited by 5 mmole Co^(2+), 5 mmole Mn^(2+), 1 mmole Ca^(2+), 0.1 mmole Na^+, 5 mmole CN^-, and 0.1 mmole CO₃^(2+) ion. But Na^+ and K^+ ion did not affect the activity of enzyme.
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